Functional characterization of the newly identified candidate targets of Urm1 conjugation in <i>Drosophila</i>.

<p><b>A.</b> Subcellular distribution of the proteins identified as Urm1-binding partners by mass spectrometry, depicting an accumulation of candidate targets of urmylation in the cytoplasmic compartment and/or associated with distinct membrane-bound organelles. <b>B.</b> Venn diagram illustrating the amount of unique versus shared Urm1-interacting proteins in the developmental stages investigated; embryos, larvae and adults. <b>C.</b> Functional clustering of the Urm1-associated proteins established using the DAVID database, suggesting that Urm1 most likely displays its most important functions in oxidation-reduction processes and tRNA modification. Enrichment scores of >3.0 were considered as meaningful. <b>D.</b> A functional classification of the Urm1-binding proteins based on gene ontology (GO) classification suggests that Urm1 may be involved in multiple different biological processes.