Additional file 13: Figure S6. of Engineering human ventricular heart muscles based on a highly efficient system for purification of human pluripotent stem cell-derived ventricular cardiomyocytes
- Bin Li (39349)
- Hui Yang (91136)
- Xiaochen Wang (235611)
- Yongkun Zhan (4480564)
- Wei Sheng (2031655)
- Huanhuan Cai (1859110)
- Haoyang Xin (4480567)
- Qianqian Liang (1521904)
- Ping Zhou (144535)
- Chao Lu (4374)
- Ruizhe Qian (124153)
- Sifeng Chen (124155)
- Pengyuan Yang (135243)
- Jianyi Zhang (104103)
- Weinian Shou (558098)
- Guoying Huang (405973)
- Ping Liang (30204)
- Ning Sun (162468)
Publication date
September 2017
DOIAbstract
Showing enrichment of human MLC-2v-positive early ventricular cardiomyocytes from hiPSCs. a, b Adding G418 after cardiac differentiation of MYL2Neo/w-hiPSCs successfully selected cardiomyocytes and markedly increased the percentage of MLC-2v-positive cells toâ~â98%. c Immunostaining also showed that, after G418 selection, MLC-2v-positive cardiomyocytes were enriched while MLC-2a-positive cardiomyocytes were almost completely removed. d FACS sorting based on GFP expression after cardiac differentiation of MYL2EGFP/w-hiPSCs successfully enriched pure MLC-2v-positive early ventricular cardiomyocytes. Almost 100% of these EGFP+ cells showed strong MLC-2v expression in cTnT-positive cardiomyocytes. (JPG 475 kb
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