Surface staining of O157 and O145 STEC strains with O157 and O145 mAbs.
- Daniela S. Castillo (4288213)
- Diego A. Rey Serantes (282100)
- Luciano J. Melli (282101)
- Andrés E. Ciocchini (282099)
- Juan E. Ugalde (252638)
- Diego J. Comerci (193090)
- Alejandro Cassola (4288216)
Publication date
October 2017
DOIAbstract
<p><b>(A-B)</b> Binding of O157 3F10 and O145 4C8 mAbs to <i>E</i>.<i>coli</i> O157 <b>(A)</b> or <i>E</i>.<i>coli</i> O145 <b>(B)</b> heat-killed bacteria assessed by flow cytometry. An irrelevant isotype-matched murine mAb or only secondary Ab were used as controls. <b>(C-D)</b> Immunostaining of <i>E</i>.<i>coli</i> O157 and O145 with O157 3F10 <b>(C)</b> and O145 4C8 <b>(D)</b> mAbs, visualized by confocal microscopy. Scale bar, 3 μm.</p
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