LLC metastasis growth in DUSP3^-/- mice is associated with the presence of higher monocytes and macrophages in lungs and with increased proliferation.

<p>Immune cell population phenotyping in LLC tumour-bearing lungs by flow cytometry. <b>(A)</b> Percentages of neutrophils, eosinophils, alveolar macrophages and monocytes/macrophages in DUSP3^+/+ and DUSP3^-/- lungs. <b>(B)</b> Absolute number of neutrophils, eosinophils, alveolar macrophages and monocytes/macrophages in DUSP3^+/+ and DUSP3^-/- lungs. <b>(C-E)</b> Monocytes/macrophages phenotype characterization in LLC tumours: Comparison <b>(C)</b> and percentage <b>(D)</b> of Ly6B^hi, Ly6B^int and Ly6B^low macrophages in DUSP3^+/+ and DUSP3^-/- mice. <b>(E)</b> Expression of specific macrophages markers in distinct Ly6B⁺ populations. Alveolar Ф = alveolar macrophages; Mo/MФ = monocytes/macrophages. Student-t-test was used for statistical analysis. *p < 0,05. 3 mice were used in each group and for each experiment. Data shown are representative of 4 different experiments.