Relative expression of NAC transcription factor in shoots of rice plants treated with 200 μM Al for 24 h.

<p><i>NAC</i> genes induced in shoots of Cotaxtla, Tres Ríos, Huimanguillo and Temporalero in response to Al treatment (A). Expression level of <i>NAC</i> genes that were differentially regulated by Al (B, C) in all rice cultivars evaluated. Relative expression of <i>NAC</i> genes that were differentially regulated by Al (C) in all rice cultivars evaluated. Relative gene expression was quantified using the comparative methods CT (threshold cycle): 2^−ΔΔCT, where ΔΔCT represents ΔCT_{condition of interest}− ΔCT_control. <i>Actin</i> (<i>Os03g50890</i>) was used as a reference gene for data normalization. The values are mean ± SE from three independent biological replicates. ma = missing annotation. Al-responsive genes identified as shoots-specific expression are highlighted in boxes with dotted lines.