Quantification of Lucifer Yellow dye transfer after various modes of GCX recovery.

<p><b>A</b>. Control <b>B</b>. With 25 μIU/ml Hep III treatment, the movement of Lucifer Yellow dye across cell junctions was impaired. <b>C</b>. Self-recovery of HS, after enzymatic degradation, allowed dye transfer across cell junctions at near baseline levels <b>D</b>. Not much Lucifer Yellow dye transfer improvement was observed after recovery of lost HS by addition of 59 μg/ml HS. Bar = 100 μm and applies to A through F. <b>E</b>. Addition of 10μM S1P also did not improve cell-to-cell communication. <b>F</b>. A combined treatment of RFPEC with both HS and S1P resulted in increase in Lucifer Yellow dye transfer; reversing the effects of enzyme treatment. <b>G</b>. Quantification of Lucifer Yellow flux across connexin-containing gap junctions in RFPEC in control, Hep III-treated, self-recovery, and artificial recovery conditions. ANOVA and TUKEY post hoc test showed statistical significance as noted in the plot and summarized in the table. The results of various treatment conditions were compared to either control or enzyme (Hep III) conditions.