Stages of migrating nucleus in the budneck before and after repression of uL4 synthesis.

<p>The Pgal-uL4B strain was modified with Pus2-RFP as a marker for nucleoplasm and Sec63-GFP as a marker for nuclear and cell membranes. (A) Stages of mitosis during growth in galactose. (B) Cells after growth in glucose for 15 hours. Mothers are associated with one post mitotic daughter surrounded by a complete plasma membrane and containing its own nucleus. A second bud has formed at each of the mother cells and the nucleus migrating to the second daughter is seen in the budneck.