Dex dose-response curves of GBM dispersal on extirpated mouse retina and brain slices.

<p>Fluorescently-labeled spheroids of GBM-2 (A) and GBM-3 (B) were generated and plated onto mouse retina or brain slices in Dex concentrations ranging from 1x10^-10 M to 1x10^-6 M. Spheroids were incubated for 24 hours and dispersal area was plotted as a function of dose. For each data set, n = 10–12, depending on outlier removal by ROUT test. Curves were analyzed by non-linear regression. R-squared values for Goodness-of-fit ranged from 0.61 to 0.81. Log EC50 values were compared for mRetina and mBrain by Extra Sum-of-Squares F test (α of 0.05). p-values for differences in Log EC50 between mRetina and mBrain were 0.0033 and 0.0011 for GBM-2 and GBM-3, respectively.