Tpk2 and Tpk3 affect the translational response to severe heat stress.

<p>(A) mRNA expression level was determined by q-RT-PCR on samples before and after severe heat stress. The value represents mean +/- SEM, n = 2. <i>ACT1</i> mRNA was used as a control. (B) The RNA collected from sucrose gradient fractions was pooled into monosome (M) and polysome (P) fractions. The mRNA distribution was analyzed by qRT-PCR and quantified relative to a luciferase mRNA control. Translational activity change was calculated as described in Materials and Methods. The values represent mean from two independent samples. The value represents mean +/- SEM, n = 2.