A semi-synthetic glycosaminoglycan analogue inhibits and reverses <i>Plasmodium falciparum</i> cytoadherence - Fig 6

<p>A) Prothrombin time (PT) assay to determine EC₅₀ for the compounds for coagulation. 100% represents the inhibition of clotting, determined as a PT of >120 seconds, 0% represents a normal PT clotting time for pooled human plasma (≈ 13–14 seconds). EC₅₀ Porcine Mucosal Heparin (PMH, the clinically used antihrombotic agent) = 2.74 x 10⁻¹ μg.mL^-1; EC₅₀ GSII = 5.44 x 10⁴μg.mL^-1. B) Activated partial thromboplastin time (aPTT) assay. 100% represents the inhibition of clotting, determined as a aPTT of >120 seconds, 0% represents a normal PT clotting time for pooled human plasma (≈ 35 seconds). EC₅₀ Porcine Mucosal Heparin (PMH) = 6.74 x 10⁻¹ μg.mL^-1; EC₅₀ PACS = 1.53 x 10⁴ μg.mL^-1. Data for Fig 6 are provided in supporting information in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186276#pone.0186276.s011" target="_blank">S6 Data</a>.