Performing functional connectivity analysis in the awake mouse brain.

<p>Time series of spontaneous GCaMP6 activity filtered over the canonical functional connectivity band: (A) 0.009–0.08H, (B) 0.08–0.4Hz, and (C) the delta band: 0.4–4.0Hz. Corresponding functional connectivity maps constructed using a seed from left motor (Mot.) cortex (black circle) in 0.009–0.08Hz (top right), 0.08–0.4Hz (center right), and 0.4–4.0Hz (bottom right) spontaneous data are also shown. Black and red traces correspond to left and right motor cortices, respectively. The green trace is sampled from right cingulate cortex. Over both frequency bands, left and right motor cortex show highly correlated activity whereas right motor with left cingulate show reduced temporal correlation. At the pixelwise level, correlating the time series from the left motor (black) with every pixel in the brain produces a map showing homotopic functional somatosensory structure across hemispheres. Mot.: Motor, Cing.: Cingulate.