Comparison of the adipogenic differentiation potentials of hTMSCs cultured in serum-free medium versus those of hTMSCs cultured in serum-containing medium.

<p>Under adipogenic conditions, adipogenesis was perceived after 2 weeks of culture by staining of intracytoplasmic microvacuole with Oil Red O. Visual assessment revealed similar levels of intracytoplasmic Oil Red O staining (x 400) in hTMSCs cultured under serum-free medium (left) and in hTMSCs cultured under serum-containing medium (right). The mRNA expression levels of peroxisome proliferator activated receptor r (PPARr) and AcylCoA synthetase (ACS) in hTMSCs (B) were detected by RT-PCR. hTMSCs cultured under serum-free medium exhibited higher expression of adipogenic differentiation markers compared with hTMSCs cultured under serum-containing medium.