Additional file 1: Table S1. of Chronic hepatitis B carriers with acute on chronic liver failure show increased HBV surface gene mutations, including immune escape variants

Clinical and virological data of 12 patients with Chronic-Hepatitis B (CHB)-associated Acute-on-Chronic Liver Failure (ACLF). Table S2. The substitutions between aa 100–200 region of HBV S gene in plasma from in Chronic Hepatitis B patients with Acute/Chronic Liver Failure. Table S3. Summary of classic IE mutations and list of classic IE mutations and other mutations in “a determinant” region of HBV S gene found in Chronic Hepatitis B patients with Acute/Chronic Liver Failure (N = 7). Figure S1. Comparison of HBV P/S and BCP/PC sequences diversity in CHB patients with and without Acute-on-Chronic Liver Failure (ACLF). (a) Analysis of HBV P/S sequence diversity in plasma among different groups. The viral diversity of P/S sequences was comparable between each group. (b) Analysis of HBV BCP/PC sequence diversity in plasma of different groups. There was no significant difference in HBV diversity of BCP/PC gene observed among distinct groups. Figure S2. Alignment of HBV S gene consensus sequences within the “a” determinant region to HBV sequences from a cohort of chronic HBV patients with Acute/Chronic Liver Failure (N = 7). The classic IE mutations in the patients were indicated by red arrows while other substitutions are noted by black arrows. Both classic IE-associated and other substitutions in “a” determinant are common among these patients. A specific list of substitutions for each patient is provided in Additional file 1: Table S3. (DOCX 588 kb