TW-37 provokes apoptosis in CRC cells.

<p>HCT-116 CRC cells (A-D), the primary human colon cancer cells (two lines, “Colon can-1/2”, E) and the primary human colon epithelial cells (“Colon Epi1”, E) were either left untreated (“C”) or stimulated with TW-37 at applied concentrations (10–1000 nM), cells were further cultured in TW-37-containing-medium for designated time; Cell apoptosis was tested by the listed assays mentioned in the text. Cleaved-Caspase-9 and Cleaved-PARP were quantified and normalized to β-Tubulin (B). Data were presented as mean (n = 5) ± standard deviation (SD). *<i>p</i><0.05 vs. “C”. Experiments in this figure were repeated three times, and similar results were obtained.