Effects of topical T-3833261 or Halofuginone on TGF-β-induced fibrotic genes expression in mouse ear skin.

<p>TGF-β (500 ng/20 μL) or vehicle was intradermally injected 5 times in the ear of mice. T-3833261, Halofuginone or vehicle was locally administered to the mice 1 h before TGF-β injection. Skin biopsies were taken from each sample treated mice. mRNA expression of α-Sma, Col1a1 and Col1a2 in the ear was measured 78 h after final TGF-β administration was evaluated. Gene expression levels (normalized to Gapdh) are expressed as the fold change of vehicle-treated control. Values are mean ± SE (n = 4–8). #p<0.05 compared to PBS-injected normal mice, *p<0.05 compared to TGF-β-injected mice.