Representative dot plots histograms of the experimental setup and analysis of flow cytometry data.

<p>(A) Dot plot histogram for entire cell population after isolation. (B) Dot plot histogram of single cells population according to FSC-H (H- high) vs. FSC-A (A- area scaling). (C) Representative histogram of negative single cells population for Fixable Viability Dye eFluor 780 (viable cells, live/dead); (N = 6 pial, N = 4 parenchymal and N = 4 retina). (D) Representative histogram demonstrating two subpopulations of SM22α-positive events of viable cells suspension (log scale) (N = 5, *p˂0.05 pial, N = 4 parenchymal and N = 3 retina). (E) Representative histograms demonstrating ET_B-positive events (log scale); (N = 5 pial, N = 4 parenchymal and N = 3 retina). The green line indicates the IgG for SM22α or ET_B respectively. The analysis was performed from three tissue types: 1- pial vessels, 2- cerebral parenchymal vessels and 3- retina vessels.