Fluorescence emission spectra of ThT-stained aggregates obtained through mixing of MA and HEWL solutions in D₂O.

<p>Complexes were prepared by mixing samples at pD 7.6 and 21:1 (A) and 1:1 (B) MA:HEWL molar ratios. Prior to measurements, aggregates were incubated at 65^°C / 300 rpm for specified periods of time (RT denotes spectra of freshly prepared samples). Inset in panel B shows emission spectra of ThT-stained HEWL samples in the absence of MA. All spectra shown were collected for identical ThT and protein concentrations, and using identical optical pathways and photomultiplier gain (voltage) settings; λ_exc. = 440 nm.