siRNAs for E6 and E7 silencing are functional from Upregulation of PIR gene expression induced by human papillomavirus E6 and E7 in epithelial oral and cervical cells
- Diego Carrillo (715802)
- Juan P. Muñoz (715803)
- Hernán Huerta (4556515)
- Gabriel Leal (4556509)
- Alejandro Corvalán (44828)
- Oscar León (715805)
- Gloria M. Calaf (4556518)
- Ulises Urzúa (3495770)
- Enrique Boccardo (94411)
- Julio C. Tapia (4556512)
- Francisco Aguayo (45572)
Publication date
October 2017
DOIAbstract
(a, b) The usefulness of siRNAs and scrambled for HPV-16 E6 and E7 silencing was evaluated by RT-qPCR. (c) Immunofluorescence by confocal microscopy for HPV-16 E7 oncoprotein in Ca Ski cells transiently transfected with siRNA against E7 or scrambled. Results are representative of three independent experiments
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