Naive CD4 T cells activated in non-polarizing conditions in vitro show pattern similar to memory cells generated in vivo.

<p>(A) Dose response curve for primed and recalled NCD4 T cells from B6 mice from one experiment to show secreted cytokine levels in primed and recalled cells. Data representative of >5 independent experiments. Cytokine values for naive cells were <10 pg/ml. (B) A representative profile to show T-bet and GATA-3 levels in naive and in vitro primed CD4 cells from B6 mice. (C) Data from 7 independent experiments compiled to show relative MFI values (fold increase over isotype control) for T-bet and GATA-3 (mean ± s.e.) from B6 mice. (D) Data from 5 sets of independent experiments compiled to show relative mRNA levels, normalized to NCD4T cell values, 72 h after priming (mean ± s.e.). (E) Dual color ELISpot assay to show number of spots (mean ± s.e.). Data representative of 4 independent experiments on B6 T cells. (F) Primed NCD4T cells from B6 mice show large presence of IFNg producing cells after P+I treatment. (G) Data from independent experiments on NCD4T cells from B6 mice compiled to show relative T-bet and GATA-3 MFI increases over naive cells in IFNg+ and IL-13+ cells (mean ± s.e.). (H) NCD4 T cells from BALB/c mice primed for 72 h in vitro and recalled to score single and dual cytokine producing cells. Frequency of cytokine producing cells from naive and primed T cell groups (mean ± s.e., n = 3).