Additional file 9: Figure S2. of Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants

Rarefaction curves 16S rRNA gene sequencing data. Rarefaction curves representing number of species (leaves) detected at genus level versus number of reads sampled. Three different 16S rRNA gene sequencing data were used for this study: i) green curves represent sequencing data from 16S rRNA library prepared using primers 27F-519R, ii) red curves represent sequencing data from 16S rRNA library prepared using primers 530F-926R and iii) blue curves represent sequencing data from 16S rRNA library prepared using primers 926F-1394R. Rarefaction curves are labelled with numbers to differentiate among the samples used in the study: 1 (V2A.530F), 2 (V2A.926F), 3 (V3 J.926F), 4 (AP8C.530F), 5 (V2AJ.27F), 6 (AP5D.530F), 7 (P35C.530F), 8 (V3 J.530F), 9 (P29F.530F), 10 (P31B.530F), 11 (P30N.530F), 12 (AP25E.530F), 13 (AP25E.926F), 14 (AP25E. 27F), 15 (AP8C.926F), 16 (P35C.926F), 17 (V3 J.27F), 18 (P31B.926F), 19 (AP1E.530F), 20 (P31B.27F), 21(P29F.27F), 22 (P30N.27F), 23 (AP5D.926F), 24 (AP5D.27F), 25 (P29F.926F), 26 (AP1E.926F),27 (AP1E.27F), 28 (AP8C.27F), 29 (P30N.926F), 30 (P35C.27F). Numbers 12, 13 and 14 correspond to sample AP25E where majority of sequenced reads assigned at family level (i.e. Enterobacteriaceae). (PDF 62 kb