Lactate production from HeLa cells exposed to 2-deoxy-D-glucose (2DG) or non-radioactive 2-deoxy-2-fluoro-D-glucose (¹⁹FDG) under either normoxic or hypoxic (1% O₂) conditions.

<p>HeLa cells were treated with different doses of 2DG or ¹⁹FDG for 24 hours under either normoxia (A) or hypoxia (B), and lactate production was measured from the cell culture media. Lactate production was normalized to the amount of protein from the corresponding cells. n = 3, * denotes significance from 2DG within the same glucose analog concentration, p≤0.05. All doses of both analogs significantly decreased lactate production of cells compared to untreated cells.