MOESM1 of The HIV-1 integrase-LEDGF allosteric inhibitor MUT-A: resistance profile, impairment of virus maturation and infectivity but without influence on RNA packaging or virus immunoreactivity

Additional file 1. Supplementary methods. Chemical synthesis process of MUT-A,HTRF®-based IN-LEDGF interaction assay, HTRF®-based IN multimerization assay, Viral RNA isolation, Northern blot analyses, primer extension assays, RT activity assays, Cryo-electron microscopy of HIV particles, Determination of HIV-1 replication capacity. Figure S1: 1H NMR spectrum of MUT-A. Figure S2: Impact of MUT-A on HIV-1 replication and production. Figure S3: Analysis of genomic RNA, Reverse transcriptase and tRNALys3 primer in MUT-A-treated HIV-1. A-D. HIV-1 RNA packaging and thermal stability of HIV-1 RNA dimers. Figure S4: HIV-1 NL4-3 particles observed by cryo-EM. A. Figure S5: Replicative capacity of HIV-1 NL4-3 viruses bearing resistance mutations to INLAIs or INSTIs. Table S1: Immunoreactivity of HIV-1 NL4-3 produced in the presence of MUT-A, or Saquinavir, or after AT2 treatment, or in the presence of DMSO. Figure S6: Results of virus immunocapture from Table S1 represented in bar graphs