<p>(A) Schematic representation of SDC4 clones. The Ser179 of the cytoplasmic domain of SDC4 was mutated to non-phosphorylatable Ala (Ser179Ala) or, phosphomimetic Glu (Ser179Glu), respectively. The type II PDZ binding site was deleted in the Ser179 mutants generating ΔPDZ constructs. (B) Co-IPs were carried out with anti-Tiam1 antibody from the lysates of the different SDC4 lines and the subsequent immunoblot was probed with anti-GFP-HRP (GFP::SDC4) or anti-Rac1 antisera, respectively. Tiam1, Rac1 and GFP::SDC4 were used as loading controls. The strongest interactions were detected in the Ser179Glu and ΔPDZ-Ser179Glu cell lines. The Rac1 was greatly reduced in Tiam1 co-IP in the lysates of Ser179Glu cells only. The bar diagrams show the re...
<p>The mutant Tiam1 binding site was generated in the complementary site for the seed region of miR-...
<p>(A) Rac1 GTP loading was measured by biotinylated Pak-CRIB peptide-based pull-down (PD) with lysa...
<p>Overexpression of Myc-tagged MAP1B constructs in COS7 cells and N1E115 cells was analyzed by immu...
<p>(A) The only connection of SDC4 to Tiam1 via PDZ binding site or Glu<sup>179</sup> does not inter...
The small GTPases of the Rho family comprising RhoA, Rac1 and Cdc42 function as molecular switches c...
<div><p>The small GTPases of the Rho family comprising RhoA, Rac1 and Cdc42 function as molecular sw...
The small GTPases of the Rho family comprising RhoA, Rac1 and Cdc42 function as molecular switches c...
<p>(A) The level of active Rac1-GTP was monitored in MCF-7 cells expressing different SDC4 construct...
<p>Schematic representation showing the upstream and downstream effects of Tiam1 in intracellular pa...
Tiam1 encodes an exchange factor for the Rho-like guanosine triphosphatase Rac. Both Tiam1 and activ...
Tiam1 (T-lymphoma invasion and metastasis 1) regulates migra-tion, cell–matrix and cell–cell adhesio...
Cell polarity is an essential requirement for the proper tissue development of complex organisms. Th...
Tiam1 is one of the most extensively analyzed activators of the small GTPase Rac. However, fundament...
Tiam1 (for T-lymphoma invasion and metastasis-inducing protein) belongs to the Rho GEF family of pro...
The exchange factor Tiam1 regulates multiple cellular functions by activating the Rac GTPase. Active...
<p>The mutant Tiam1 binding site was generated in the complementary site for the seed region of miR-...
<p>(A) Rac1 GTP loading was measured by biotinylated Pak-CRIB peptide-based pull-down (PD) with lysa...
<p>Overexpression of Myc-tagged MAP1B constructs in COS7 cells and N1E115 cells was analyzed by immu...
<p>(A) The only connection of SDC4 to Tiam1 via PDZ binding site or Glu<sup>179</sup> does not inter...
The small GTPases of the Rho family comprising RhoA, Rac1 and Cdc42 function as molecular switches c...
<div><p>The small GTPases of the Rho family comprising RhoA, Rac1 and Cdc42 function as molecular sw...
The small GTPases of the Rho family comprising RhoA, Rac1 and Cdc42 function as molecular switches c...
<p>(A) The level of active Rac1-GTP was monitored in MCF-7 cells expressing different SDC4 construct...
<p>Schematic representation showing the upstream and downstream effects of Tiam1 in intracellular pa...
Tiam1 encodes an exchange factor for the Rho-like guanosine triphosphatase Rac. Both Tiam1 and activ...
Tiam1 (T-lymphoma invasion and metastasis 1) regulates migra-tion, cell–matrix and cell–cell adhesio...
Cell polarity is an essential requirement for the proper tissue development of complex organisms. Th...
Tiam1 is one of the most extensively analyzed activators of the small GTPase Rac. However, fundament...
Tiam1 (for T-lymphoma invasion and metastasis-inducing protein) belongs to the Rho GEF family of pro...
The exchange factor Tiam1 regulates multiple cellular functions by activating the Rac GTPase. Active...
<p>The mutant Tiam1 binding site was generated in the complementary site for the seed region of miR-...
<p>(A) Rac1 GTP loading was measured by biotinylated Pak-CRIB peptide-based pull-down (PD) with lysa...
<p>Overexpression of Myc-tagged MAP1B constructs in COS7 cells and N1E115 cells was analyzed by immu...