Additional file 1: Figure S1. of Bi-directional exosome-driven intercommunication between the hepatic niche and cancer cells

Relative absolute values of the miRNA species are provided for comparison to each other coming from the bioreactor with and without MDA-231 cells. Figure S2. Relative absolute values of the miRNA species are provided for comparison to each other coming from the MDA-231 cells in 3D tissue versus 2D. Figure S3. A upper panel: Experimental outline for cell cycle analysis. A lower panel: Cell cycle analysis, using Propidium Iodide, of 2D co-culture experiments, following priming of the hepatic niche with cancer derived exosomes. B: miRNA content of exosomes derived from the HepN in the Liver MPS. C: IPA analysis of the miRNA content in the exosomes described above. Figure S4. Relative absolute values of the miRNA species are provided for comparison to each other coming fromMDA-231 cells after treatment with HN exosomes. Figure S5. Western blot analysis for ZO-1 protein levels on the breast cancer lines MDA-231 and MDA‐468 and the prostate cancer line DU-145 that were treated exosomes derived from human hepatocytes, NPCs and Hep/NPCs for 48 h. Cell prolifera8on of MDA-231 cells treated with exosomes derived from human hepatocytes, and Hep/NPCs for 48 h, quan8fied by trypan blue. Transwell migration of MDA-231 cells treated with exosomes derived from human hepatocytes, and Hep/NPCs for 48 h. (PPTX 540 kb