Pilocarpine-induced seizures reduce <i>KCNMB4</i>-EGFP reporter expression.

<p>(A) Anti-EGFP (top panels) and anti-MAP2 (middle panels) immunofluorescence staining from heterozygous mice containing the EGFP cDNA that replaces the first coding exon of <i>KCNMB4</i> gene [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188064#pone.0188064.ref010" target="_blank">10</a>]. The pictures are oriented from left, medial (the dentate gyrus) to right, temporal (hilar mossy fibers extending to CA3 region). Bottom panels are merged of above images. (B) Average fluorescence intensity of EGFP normalized to MAP2 measured at the granule cell area. Control is 4.1 ± 0.20, N = 7, pilocarpine 3.4 ± 0.18, N = 6, P = 0.034.