<p><b>A.</b> Representative confocal images of HBEC co-stained by cell markers CK13 (mucous cells, left panel, red labelling) and Muc5AC (ciliated cells, middle panel, red labelling) and GnRH-R (green labelling). Co-distribution of GnRH-R (green labelling) and CFTR (red labelling) was observed in ciliated cells, in apical cell membranes. <b>B.</b> Representative immunoblot performed to assess GnRH-R expression in 6 different HBEC cultures.</p
<p>Stain separation by color deconvulation method. Neurones are stained for GnRH by immunohistochemi...
<p><b>A</b>) Strategy of the screen to identify ciliary GPCRs. We constructed plasmids expressing 13...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence-arcuate (ME...
<p><b>A.</b> The upper left panel shows representative PCR bands for GnRH-R (319 bp) after separatio...
<p>Cells expressing FLAG-GnRH-RI (HX and X) were subjected to indirect immunofluorescent staining fo...
<p>GH (green) positive cells (A); OB-Rb (red) positive cells (B); and merged images for GH and OB-Rb...
<p>Example images from dual-label immunocytochemistry to detect GnRH (green, left panel), DCC (red, ...
<p>A) Light micrographs of the GnRH-R-ir cells, detected with polyclonal antibodies as described in ...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence (ME) region...
<p>The presence of CFTR within membranes was detected by biotinylation experiments. <b>A.</b> Repres...
Three gonadotropin releasing hormones, seabream GnRH (GnRH-I), chicken GnRH-II (GnRH-II) and salmon ...
The mammalian type I gonadotropin releasing hormone receptor (GnRH-R) is a structurally unique G pro...
<p>A) Light micrographs of GnRH-ir cells, detected with polyclonal antibody as described in <a href=...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence (ME) region...
<p>(<i>A</i>) Double-immunofluorescence staining showing colocalization between anti-GPR40 staining ...
<p>Stain separation by color deconvulation method. Neurones are stained for GnRH by immunohistochemi...
<p><b>A</b>) Strategy of the screen to identify ciliary GPCRs. We constructed plasmids expressing 13...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence-arcuate (ME...
<p><b>A.</b> The upper left panel shows representative PCR bands for GnRH-R (319 bp) after separatio...
<p>Cells expressing FLAG-GnRH-RI (HX and X) were subjected to indirect immunofluorescent staining fo...
<p>GH (green) positive cells (A); OB-Rb (red) positive cells (B); and merged images for GH and OB-Rb...
<p>Example images from dual-label immunocytochemistry to detect GnRH (green, left panel), DCC (red, ...
<p>A) Light micrographs of the GnRH-R-ir cells, detected with polyclonal antibodies as described in ...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence (ME) region...
<p>The presence of CFTR within membranes was detected by biotinylation experiments. <b>A.</b> Repres...
Three gonadotropin releasing hormones, seabream GnRH (GnRH-I), chicken GnRH-II (GnRH-II) and salmon ...
The mammalian type I gonadotropin releasing hormone receptor (GnRH-R) is a structurally unique G pro...
<p>A) Light micrographs of GnRH-ir cells, detected with polyclonal antibody as described in <a href=...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence (ME) region...
<p>(<i>A</i>) Double-immunofluorescence staining showing colocalization between anti-GPR40 staining ...
<p>Stain separation by color deconvulation method. Neurones are stained for GnRH by immunohistochemi...
<p><b>A</b>) Strategy of the screen to identify ciliary GPCRs. We constructed plasmids expressing 13...
<p>Confocal immunofluorescent images of 30 µ thick coronal sections from median eminence-arcuate (ME...