Relative telomerase activity measurement of hADSCs in the presence of different concentration of ZnSO₄ for 48 hours of incubation as detected by the PCR-ELISA TRAP assay.

<p>Cells were seeded at a density of 5×10⁴ cells/wells for about 48 hours in the presence of 1.5×10⁻⁸ and 2.99×10⁻¹⁰ M ZnSO₄. Following, cells were lysed and protein were extracted from each sample. Relative telomerase activity was assessed in protein extracts by Telomerase PCR-ELISA kit. Heat inactivated cell extract served as the negative control. As described in results section, 1.5×10⁻⁸ M ZnSO₄ was significantly increased the relative telomerase activity of hADSCs (*P<0.05 compared with control group). This procedure was repeated for three times. Data represent as the means ± SE from three independent biological experiments. One-way ANOVA followed by Dunnett’s post hoc test was used to determine the significant difference among groups.