AQP2 acute response to vasopressin differed in slices of inner medulla from wild type and <i>Fxyd1</i> knockout mice.

<p>Kidney slices from WT (a-c) and KO (d-f) male mice were fixed without treatment (a,d), incubated in HBSS to wash out endogenous VP (b,e), and then treated with dDAVP for 15 min (c,f). Slices were post-fixed with 2% PLP and sectioned. The distribution of AQP2 was monitored by immunofluorescence. The absence of FXYD1 reduced shuttling of AQP2 between intracellular vesicles and subapical spaces and apical membrane. This figure is representative of 3 independent experiments. Nuclei were labeled with Dapi. Bars, 10 μm.