Additional file 10: Figure S7. of msaABCR operon is involved in persister cell formation in Staphylococcus aureus

Continuous monitoring of biofilm formation in the presence of vancomycin. Stains USA300 LAC, msaABCR deletion mutant and the complementation were inoculated into the BioFlux microfluidic system and allowed to form a biofilm for 12 h. Vancomycin (12.50 μg/ml, 20× MIC) treatment was started and 0-h time indicated the point of antibiotic treatment. The flow rate of media with vancomycin was set at 64 μl/h and biofilm was monitored up to 50 h. Bright-field images were collected every 10 m intervals using an inverted Leica DM IL LED Fluo Inverted Microscope under 5X magnification. a. Area coverage of biofilm. b. Representative images of biofilm development at different time points (TIFF 9042 kb