Anti-FMM molecules affects membrane organization of FloA foci.

<p>(A) Effect on growth of different concentrations of anti-FMM molecules simvastatin (SIM), zaragozic acid (ZA) or 5-doxyl-stearic acid (5-DSA) in TSB medium. (B) Fluorescent foci in FloA-MARS-labeled cells after treatment with 20 μM SIM, 150 μM 5-DSA or 50 μM ZA compared to untreated cells. (C) Immunoblot analysis of membrane and cytosol fractions of wild type cells treated with 20 μM SIM, 150 μM 5-DSA or 50 μM ZA. Untreated cells served as control. Flotillin was detected using polyclonal anti-FloA antibody.