NRTN gene transfection increases TH(+) fibers in the striatum of rats with chronic 6-OHDA lesion.

<p><b>A.</b> Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188239#pone.0188239.ref054" target="_blank">54</a>]. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. <b>B.</b> Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * <i>P</i> < 0.001, ** <i>P</i> < 0.0001. ns = no statistical significance, <i>P</i> > 0.05.