Immunohistochemical analysis of cells migrating out of ischemic retinal explants.

<p>Retinal explants were transferred onto laminine-1 coated cultures for six days to allow RGC regeneration. Next, explants were analyzed by immunohistochemistry. Different cell types migrated out of the retinal explants during the time in culture. <b>(A+E)</b> Two types of DAPI-positive nuclei were apparent: smaller (circle) and bigger (asterisk). <b>(B+D)</b> The smaller nuclei belonged to iba-1 positive microglia cells (circle). <b>(F+H)</b> The bigger nuclei belonged to GFAP-positive Müller cells and astrocytes (asterisk). <b>(C+G)</b> Both glial cell types did not compromise the growth behavior of the beta-III tubulin positive RGC axons. Scale bar upper row 300 μm, lower row 600 μm.