Effects of primary antibody concentrations on sensitivity in M10 ELISA.

<p>A series of primary antibody dilutions (1:500, 1:600, 1:700, 1:800, 1:900 and 1:1000) in PBS were tested against 1:10,000 (0.2 μg /ml) concentration of detection antibody. Each data point represents the mean value of the corresponding triplicates.