<i>T</i>. <i>cruzi</i> infection increases glucose uptake by host cells.

<p><b>(A)</b> Uptake of [³H]-2-deoxyglucose ([³H]-2-DG) into uninfected or <i>T</i>. <i>cruzi</i>-infected NHDF monolayers at 48 hours post infection (48 hpi), in which infection was established with a varying multiplicity of infection (MOI). Mean ± SD shown for 3 biological replicates per MOI. One-way ANOVA with Dunnett’s multiple comparison test was applied for individual comparisons to the uninfected control group (*p<0.05, ****p<0.0001). <b>(B)</b> Cytochalasin B (10 μM) blocks uptake of [³H]-2-DG in uninfected and infected NHDF monolayers (48 hpi). Mean ± SD shown for 3 biological replicates. Two-way ANOVA with Tukey’s multiple comparisons test was applied (****p<. 0001)<b>. (C)</b> [³H]-2-DG uptake by NHDF or <b>(D)</b> C2C12 myoblasts following a 48 h infection with <i>T</i>. <i>cruzi</i> Tulahuén, CL Brener or CL-14 strains. NHDF were infected for 2 hours with MOI 40 for Tulahuén strain and MOI 150 for CL Brener and CL-14 strains. C2C12 were infected for 2 hours with MOI 80 for Tulahuén strain and MOI 150 for CL Brener and CL-14 strains. Mean ± SD for 3 biological replicates. One-way ANOVA with Dunnett’s multiple comparison test was applied for individual comparisons to the uninfected control group (**p< 0.01, ***p< 0.001, ****p< 0.0001).