Involvement of mitochondrial apoptotic pathway in LA-12/cisplatin and TRAIL combination-induced DU 145 cell killing.

<p>(<b>A</b>) The level of Bid/tBid, Noxa, and Bim in DU 145 cells after pretreatment (24 h) with LA-12 (2.5 μM) or cisplatin (5 μM) and subsequent treatment (4 h) with TRAIL (5 ng/ml), detected by Western blotting. (<b>B, C</b>) Percentage of DU 145 cells with decreased mitochondrial membrane potential (MMP, TMRE assay, flow cytometry) and active Bak protein (flow cytometry) following treatments specified in A. Results are means + S.E.M. of at least 3 independent experiments. Statistical significance (P < 0.05, * vs. control, † vs. TRAIL, □ vs. appropriate platinum drug). <b>(D)</b> The level of Bak, Bcl-2, Bcl-x_L, and cleavage of XIAP in DU 145 cells treated as in A, detected by Western blotting. The β-actin served as a loading control.