Additional file 3: Figure S3. of Obesity reversibly depletes the basal cell population and enhances mammary epithelial cell estrogen receptor alpha expression and progenitor activity

Obesogenesis in response to a high-fat diet with respect to timing and mouse strain. A Eight-week-old C57Bl/6 mice (B6-8 wk) were fed a high-fat diet (HFD) or control diet (Con). Weight gain was determined for 17 weeks (n = 7 mice/group; mean ± s.e.m.). Differences were determined using two way ANOVA. Eight-week-old FVB/N female mice (FVB-8 wk) were fed a HFD or Con for 14 weeks (n = 5 mice/group). Body weight (B) and weight gain (C) were measured (mean ± s.e.m.). No differences were detected using two way ANOVA. D Inguinal gland weight from FVB-8 wk mice fed either HFD or Con. E Quantification of tertiary branching in whole mounts from HFD and Con inguinal mammary glands from FVB-8 wk mice. F Three-week-old C57Bl/6 mice (B6-3 wk) were fed a HFD or Con. Weight gain was determined for 17 weeks (n = 9 mice/group; mean ± s.e.m.). G Fat surrounding the uterus (gonadal fat) was weighed for B6-3 wk Con and Mice fed HFD (n = 9 mice/group). H Quantification of adipocyte diameters from mammary glands of B6-3 wk Con and HFD mice (n = 9 mice/group). I Quantification of crown-like structures (CLS) from mammary glands of B6-3 wk Con and HFD mice (n = 9 mice/group). No CLS were observed in the mammary glands of Con mice. J 3-week-old FVB/N mice (FVB-3 wk) were fed a HFD or Con diet. Weight gain was determined for 17 weeks (n = 9 mice/group; mean ± s.e.m.). K Gonadal fat was weighed for FVB-3 wk Con and HFD mice (n = 9 mice/group). L Quantification of adipocyte diameters from mammary glands of FVB-3 wk Con and HFD mice (n = 9 mice/group). M Quantification of CLS from mammary glands of FVB-3 wk Con and HFD mice (n = 9 mice/group). No CLS were observed in the mammary glands of Con mice. Bars represent mean ± s.d. (PDF 151 kb). (PDF 151 kb