V5-tagged Shb is specifically detected with an anti-V5 antibody.

<p>The V5 signal corresponding to Shb-V5 was detected by western blot. HEK 293T cells were transfected with empty plasmid or a Stag-Shb-V5 encoding plasmid. To test the specificity of the signals, the CE12 cell line bearing an endogenous V5-tagged Shb allele was knocked-down for Shb (siRNA^Shb) or treated with control siRNA (siRNA^SCR). Arrowheads point to Shb-V5. Equal amounts of proteins were loaded (50 μg) on each lane.