Additional file 3: Figure S2. of Selective proliferative response of microglia to alternative polarization signals

In vitro proliferative and polarization responses of macrophages to IL-4, CSF-1 and E2. A) The expression of proliferation (Ki67, Cdk1 and Ccnb2) and M2 polarization (Arg1, Fizz1, Mrc1) genes was analyzed in primary cultures of peritoneal macrophages following treatment with 20 ng/ml IL-4 (black bars) or 20 ng/ml CSF-1 (gray bars). B) The expression of proliferation (Ki67, Cdk1 and Ccnb2) and M2 polarization (Arg1, Vegfα) genes was analyzed in primary cultures of peritoneal macrophages following vehicle (0.01% EtOH) or 10−5 M E2 treatments. Bars represent the mean ± SEM of 3 independent experiments, each performed in triplicate Data sets for each gene were calculated using the 2-ddCt method with respect to the mean value of the vehicle group. Bars represent mean values ± SEM (n = 3). Student’s unpaired t-test, *p < 0.05; ***p < 0.001. (PDF 339 kb