Additional file 2: Figure S1. of Selective proliferative response of microglia to alternative polarization signals

In vitro polarization responses of microglia to IL-4 and CSF-1. A) The expression of M2 polarization genes (Arg1 and Mrc1) was analyzed in primary cultures of microglia following the treatment with vehicle (veh; open boxes), 20 ng/ml IL-4 (closed boxes) or 20 ng/ml CSF-1 (gray boxes). B) The expression of IL-4Rα was analyzed either in microglia cells obtained by immunosorting from the brain of vehicle or IL-4 icv-injected mice (ex vivo) or in primary cultures of microglia (in vitro) as specified above. Bars represent the mean ± SEM of 3 independent experiments, each performed in triplicate. Data sets for each gene were calculated using the 2-ddCt method with respect to the mean value of the vehicle group. Bars represent mean values ± SEM (n = 3). Student’s unpaired t-test, *p < 0.05; **p < 0.01; ***p < 0.001. (PDF 167 kb