Effect of IQGAP1 knock down on cell morphology and cell division.

<p>Native B16F10 mouse melanoma cells (F10), virus control cells (F10 VC), IQGAP1 knock down cells (F10 KD) and F10 KD cells transfected with GFP-IQGAP1-FL (F10 rKD) were incubated on laminin-coated glass coverslips for 45 min (A-D) or in 12 well TCP plates for 4 d (E). (A) Representative images show individual nucleus, actin, IQGAP1 channels with combined nucleus (blue) and actin (green) images. The arrowhead marks a cell expressing GFP-IQGAP1. B) IQGAP1 protein quantified through immunofluorescence intensity. n = 38–40 cells for each condition. C) Projected cell area from actin images. n = 38–44 cells for each condition. D) Cell length expressed as the longest chord through the cell measured from actin images. n = 38–44 cells for each condition. E) Number of cells per cm² at 0, 1, 2, 3 and 4 d. n = 9 wells over 3 experiments for each time point. ***p<0.001 Tukey’s post hoc test as indicated by the brackets in C and D, or comparing F10 KD to F10 and F10 VC in B and E. Bars = mean +/- s.e.m. Time points = mean +/- 1 s.d.