Stress-activated regulators show both coordinated and decoupled nuclear localization.

<p>(A) Distribution of nuclear/cytoplasmic signal for paired factors in individual cells before and after NaCl treatment (average <i>n</i> = 676 cells per time point). Data from two biological replicates were very similar and combined (<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004050#pbio.2004050.s011" target="_blank">S11 Table</a>). (B) Median ratios from (A) plotted over time; the Msn2 plot combines measurements from all three strains. (C) Nuclear TF signals (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004050#sec013" target="_blank">Methods</a>) of Dot6-GFP (left) and Msn2-mCherry (right) expressed in the same cells over time, before stress and after NaCl addition at 81 min (arrows). Each row aligned across all plots represents a different cell, and each column represents a different time point. Red plots show traces of nuclear localization according to the key (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004050#sec013" target="_blank">Methods</a>), and corresponding grey-scale plots show quantitative measurements only for time points called as peaks. Colored boxes above the plots indicate 80 min before stress (grey box), 30 min after NaCl treatment (dark red box), and beyond 30 min after NaCl treatment (pink box). Data are available in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004050#pbio.2004050.s012" target="_blank">S12 Table</a>. (D) Correlation between Dot6-GFP and Msn2-mCherry traces for each temporal phase, according to the key. (E) Representative traces from (C), where called peaks (colored according to key) are indicated with asterisks. TF, transcription factor.