GSK247246 promotes remyelination in cuprizone mouse model. Histopathology of corpus callosum.

<p>Black gold staining of corpus callosum (a) and cortex (b) from cuprizone mice, untreated (n = 6) or treated with vehicle (n = 9) or 30 mg/kg GSK247246 (n = 7) for 9 days prior to sacrifice(scale bar = 100 μm); p-values were generated by unpaired Student’s t-Test; (c) representative immunofluorescence image showing GST-π (red) and DAPI (blue) staining in the corpus callosum, untreated (n = 6) or treated with vehicle (n = 9) or 30 mg/kg GSK247246 (n = 7) for 9 days prior to sacrifice (scale bar = 300 μm); p-values were generated by one-way ANOVA with post-hoc Bonferroni's multiple comparisons test (F = 14.59); (d) representative immunofluorescence image showing PDGFRα (green) staining in the corpus callosum, untreated (n = 6) or treated with vehicle (n = 9) or 30 mg/kg GSK247246 (n = 7) for 9 days prior to sacrifice (scale bar = 300 μm); p-values were generated by one-way ANOVA with post-hoc Bonferroni's multiple comparisons test (F = 27.32); (e) electron microscopy of myelin and oligodendrocytes surrounding axons in the corpus callosum of cuprizone-treated mice, untreated (n = 3) or treated with vehicle (n = 5), 10 mg/kg GSK247246 (n = 5) or 30 mg/kg GSK247246 (n = 5) for 9 days prior to sacrifice (scale bar = 2 μm); p-values were generated by one-way ANOVA with post-hoc Bonferroni's multiple comparisons test (Upper (F = 5.312), Lower (F = 4.438). DAPI, 4',6-diamidino-2-phenylindole; GST-π, glutathione S-transferase π; H3R, histamine receptor-3; PDGFRα, platelet-derived growth factor receptor. *p<0.05; **p<0.01; ***p<0.005.