MOESM3 of Disruption of the c-Myc/miR-200b-3p/PRDX2 regulatory loop enhances tumor metastasis and chemotherapeutic resistance in colorectal cancer

Additional file 3: Figure S3. c-Myc represses growth, invasion and EMT of CRC cell by regulating miR-200b-3p. (a) Morphological changes of SW480 cells were observed under the microscope after overexpression of c-Myc and co-expression of c-Myc and miR-200b-3p. Scale bars represent 50 μm. (b) The number of invaded cells was counted under the microscope, with five HPF observation (*** p < 0.001). (c) SW480/Mock, SW480/c-Myc and sw480/c-Myc+miR cells (1 × 106) were subcutaneously injected into the nude mice (n = 5) for four weeks and the isolated subcutaneous tumors was observed with naked eyes. (d) HE staining for local invasion of subcutaneous tumors derived from SW480/Mock, SW480/c-Myc and SW480/c-Myc+miR cells. Red arrows point at false fibrous membrane. Scale bars represent 50 μm