Cytokine bead array (CBA) analyses of plasma samples.

<p>In duplicate wells of the 96-well filter plate, 25 μL of plasma from monkeys on days 0, 2, and 30 was incubated with 25 μL of cytokine-coupled beads overnight at 4°C, followed by washing and staining with biotinylated detection antibody. The plates were read on Biorad 200 with use of Luminex technology, and the results were expressed as pg/mL concentration. The minimum detectable concentrations in pg/mL for IFN-γ (2.2), IL-2 (0.7), IL-4 (2.7), IL-6 (0.3), IL-10 (6.2), IL-13 (5.8), IL-12/23(p40) (1.2), IL-1b (1.2), IL-ra (2.4), TNF-α (2.1), MCP1 (3.1), and VEGF (13.6) were used for considering positive responses. See the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188694#sec002" target="_blank">Materials and methods</a>” section for experimental details. P values <0.05 were considered statistically significant. <i>Symbol</i>: <i>* p<0</i>.<i>05; **p<0</i>.<i>01; *** p<0</i>.<i>0001</i>.