Representative flow cytometric gating strategy for DNA content analysis of <i>Trypanosoma cruzi</i> CL-Brener and <i>Leishmania major</i> CC1 strains stained with propidium iodide (PI).
- Lucila Langoni Naves (4694248)
- Marcos Vinícius da Silva (422813)
- Emanuella Francisco Fajardo (4694254)
- Raíssa Bernardes da Silva (4694260)
- Fernanda Bernadelli De Vito (4694257)
- Virmondes Rodrigues (499737)
- Eliane Lages-Silva (500512)
- Luis Eduardo Ramírez (4694263)
- André Luiz Pedrosa (4694251)
Publication date
December 2017
DOIAbstract
<p>Panels A and D represent relative size (FSC) and complexity (SSC). Panels B and E panels represent PI-A and PI-W parameters indicating single cells stained with PI. Panels C and F show DNA content histogram and G1-0 and G2-M peaks are pointed. Relative PI fluorescence intensity at G1-0 was used for DNA content estimation for all strains analyzed using <i>T</i>.<i>cruzi</i> CL-Brener as reference strain.</p
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