Mouse liver decellularisation.

<p>(a) Macroscopic appearance of a mouse liver scaffold during the decellularisation process. Following the perfusion with demineralized water (dH₂O), the fresh red liver becomes blanched. After perfusion with sodium deoxycholate (SDC) followed by DNAseI solution, the liver becomes transparent. (b) H&E staining of the liver sections shows no cells in the scaffold after DET decellularisation. (c) DNA quantification shows a significant (*) reduction of DNA amount in the DET liver (median = 0.093 μg/mg) compared to fresh tissue (median = 1.068 μg/mg) (n_Fresh = 4, n_DET = 4; α = 0.05; p = 0.029; two-tailed Mann-Whitney test). (d) Scanning Electron Microscopy demonstrating the preservation of the three-dimensional network of connective tissue fibres.