Verification of the effect of various glucose concentrations on cytotoxicity and <i>in vitro</i> wound healing assay in primary cultured rat gingival fibroblasts.

<p>Cytotoxicity and cell migration after incubation at various glucose concentrations were assessed in gingival fibroblasts from control rats. (A) Cytotoxicity was measured by lactate dehydrogenase (LDH) release in the supernatant. The results revealed a significant cytotoxicity with incubation at 100 mM glucose concentration. There was a tendency of increased cytotoxicity dose-dependent manner, although the difference was not statistically significant. (B) Effects of various glucose concentrations on <i>in vitro</i> wound healing assay. The relative cell migration area in the 75 mM group significantly decreased from 9 to 36 h compared with that in the 15 mM group. (C) The area under the curve (AUC) was calculated from the line graph. The AUC was the largest in the 15 mM group. The AUC of the 75 mM group was significantly smaller than that of the 15 mM group. Data are presented as mean ± SD. *<i>p</i> < 0.05 (Tukey–Kramer test). This finding was confirmed in three independent experiments.