Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors - Fig 5

<p><b>Rab8a partially mislocalizes in <i>cc2d2a-/-</i> cones</b> 5 dpf CLEM images of wild-type (wt) <b>(A-A’)</b> and <i>cc2d2a</i>-/- <b>(B-C’)</b> zebrafish expressing mCherry-tagged Rab8a in cones <i>(tg(tacp</i>:<i>mCherry-rab8a)</i>, magenta). The mCherry signal is enhanced with anti-mCherry antibody. <b>(A-A’)</b> Note the localization of Rab8a (magenta) to vesicular structures (arrowheads) in wt. <b>(B-B’)</b> In <i>cc2d2a</i>-/- cones, Rab8a (magenta) localizes to accumulated vesicles (v, bracket) as well as to non-membrane-delimited cytoplasmic areas <b>(</b>arrows in <b>C</b>-<b>C’)</b>. Scale bars are 3 μm in all panels. <i>OS</i> outer segment, <i>m</i> mitochondria, <i>N</i> nuclei, <i>v</i> vesicular structures, <i>wt</i> wild-type.