Overexpression of HSP90C affects developmental switch from skotomorphogenesis to photomorphogenesis.

<p>(A) Seeds were stratified and grown at 22°C in complete darkness for 3.5 days and then switched to constant light at 110μmol/m²s for 48h. Seedling images were taken at 0, 12, 24 and 48h after switching to light. Wild type and lines expressing different reporter proteins were indicated on the top of the seedlings. (B) Confocal fluorescence images were taken for cotyledon chloroplasts in wild type and lines expressing HSP90C^FLAG, PsbO1GFP without and with co-expression of HSP90C^FLAG. The white arrows indicate swollen and rod-like chloroplasts. (C) Purification and analysis of insoluble proteins in seedlings expressing PsbO1^1-85GFP, PsbO1GFP or co-expressing PsbO1GFP and HSP90C^FLAG. The insoluble fractions were collected after high-speed centrifugation and washed with 2% NP-40. TL, total lysates, Sol, soluble proteins, Mem, membrane bound proteins, Insol, insoluble proteins. The different fractions were loaded equivalently. Anti-HSP90C and anti-GFP were used.