Effect of boosting with Ad-lacZ on the depleted responses.

<p>(A) C57BL/6 mice were first immunized with Ad-lacZ and then >50 days later were infected with MCMV i.v. After >50 days post- MCMV infection, the mice were boosted with a second dose of Ad-lacZ i.v. The levels of CD8⁺D8V⁺ in the blood was measured by <i>ex vivo</i> tetramer staining after primary Ad-lacZ infection. The data shown are from one of two independent experiments (N = 3). (B) The distribution of the boosted cells in non-lymphoid organs were measured at day 50 after 2^nd dose of Ad-LacZ. (C) The proportion of CD44⁺ CD62L^- expression in <i>ex vivo</i> peripheral blood 6 days after primary Ad-lacZ immunization, and 6 days after second dose of Ad-lacZ. (N = 4–6 mice from 2 independent experiments). (D) The figures show the proportion of CD44⁺CD62L^- (left column) and CD27^-CD62L^- (right column) expression in CD8⁺D8V⁺ cells from the blood (upper row) and liver (lower row) of Ad-lacZ only, Ad-lacZ+MCMV and Ad-lacZ+MCMV boosted with Ad-lacZ groups, as measured by ex-vivo staining. The data are from two or more independent experiments (N = 4–11). p values were measured by two-way Anova followed by Sidak’s multiple comparison test. *p<0.05, **p<0.005.